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. 2018 Dec 21;9:3078. doi: 10.3389/fmicb.2018.03078

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Copyright © 2018 Genth, Junemann, Lämmerhirt, Lücke, Schelle, Just, Gerhard and Pich.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mass spectrometry-based evaluation of the substrate GTPase profiles glucosylated by LCGTs. Caco-2 cells were exposed to recombinantly prepared TcdB-10463 (A,B,F,G), to TcsL prepared from C. sordellii strain 6018 (TcsL-6018) (C), recombinantly prepared TcdA-10463 (D–F), and recombinantly prepared TcdB-20291 (H). Upon cell lysis, small GTPases of the Rho and Ras subfamilies were analyzed for cellular concentrations of glucosylated GTPases using MRM analysis. Glucosylation was given as the ratio of the concentration of glucosylated GTPase per concentration of total GTPase. All experiments were conducted with three biological replicates. The error bars are representing the SD of the mean.