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. 2018 Dec 21;9:3078. doi: 10.3389/fmicb.2018.03078

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Copyright © 2018 Genth, Junemann, Lämmerhirt, Lücke, Schelle, Just, Gerhard and Pich.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot-based analysis of the glucosylation of (H/K/N)Ras and Rac/Cdc42 in TcdA- and TcdB-treated Caco-2 cells. Caco-2 cells were exposed to natively prepared TcdA-10463 (A,B) or TcdB-10463 (C,D) for the indicated times. Upon cell lysis, the cellular concentrations of glucosylated (H/K/N)Ras and Rac/Cdc42 subtype GTPases were evaluated using immunoblot analysis. Immunoblots were quantified using Kodak software. The amounts of non-glucosylated Rac/Cdc42 and non-glucosylated (H/K/N)Ras relative to the respective total levels of Rac1 and H-Ras are expressed as mean ± SD of three experiments.