FIGURE 6.

Enhanced glucosylation of substrate GTPases catalyzed by rN-TcsL in the presence of the Caco-2 lysates. GST-tagged Rac1 (A,B) and GST-tagged Ras-GTPases (A,C,D) (2 μM), UDP-[¹⁴C]glucose (20 μM each) and recombinant rN-TcsL (0.3 nM) were incubated in the presence and the absence of Caco-2 lysates at 37°C for the indicated times. Upon separation on SDS-PAGE, [¹⁴C]glucosylated GTPases were visualized by autoradiography. Signals were quantified using Kodak software and are given as mean ± SD of three experiments.