Figure 2.

Overall proportion of NCBI sequences matching the 3′ region (first four bases; line type) and 5′ region (rest of the primer; x‐axis) of general COI barcoding primers. Forward and reverse primers are on top of each other, and the number of NCBI sequences on which each primer was tested is shown in parentheses beside the primer name. The newly designed forward primer (Sauron‐S878) is on the right‐hand side and combined with jgHCO2198, as was the primer mCOIintF. Note that for Sauron‐S878, mCOIintF, and jgHCO2198, there is almost no variability in fit for the 3′ end and lines are therefore overlapping