Figure 3.

In silico testing. Proportion of NCBI sequences matching (full match in the first four bases of the 3′ end and 0–3 mismatches in the remaining 5′ region; figure rows) general COI primers (figure columns) for different classes of animals with the number of NCBI sequences covering the priming site in parentheses. The newly designed forward primer (Sauron‐S878) is in the central column flanked by mCOIintF (left) and the reverse primer jgHCO2198 (right). Horizontal dashed lines show mean coverage of all NCBI sequences