FIGURE 3.

Oxytetracycline treatment alters the growth conditions provided by CLas-associated microbiota for CLas. (A) Procedures for incubation of CLas Ishi-1 among CLas-associated microbiota. CLas-infected plant materials were ground in oxytetracycline or sterilized distilled water and held for 4 h at 25°C. Each was rinsed three times and resuspended in sterilized distilled water, in which it was incubated for up to 120 h. (B) CLas Ishi-1 was not eradicated by oxytetracycline among CLas-associated microbiota but its growth suffered after the removal of oxytetracycline. CLas cells were quantified by real-time PCR of total DNA extracted from each sample. Data are means ± SD (n = 3). Statistical significance was determined by Student’s t-test (^∗P < 0.05, ^∗∗P < 0.01).