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. 2018 Oct 9;18:15–24. doi: 10.1016/j.molmet.2018.10.003

Figure 1.

Figure 1

Argonaute2 in liver is modulated according to glucose metabolism. A, Western blotting analysis of Ago2 and glucokinase (Gck) in the isolated hepatocytes of 10-week-old WT mice that were treated ex vivo with 2.5 and 25 mM glucose (n = 3). B, Quantitative RT-PCR analysis of Ago2, Ago1, Gck, G6pase, Pyruvate carboxylase (Pc1), and Phosphoenolpyruvate Caroxykinase (Pepck) in the isolated hepatocytes of 10-week-old WT mice after treatment of 2.5 and 25 mM of glucose (n = 3 for both concentrations) for 48 h. C, D, Western blotting analysis of Ago2 in the isolated hepatocytes of 10-week-old WT mice after treatment with 0, 2.5, or 25 mM 2-deoxyglucose (2-DG) for 16 h (n = 3). E, Western blotting analysis of Ago2 in the isolated hepatocytes of 10-week-old WT mice after receiving 0 or 25 mM glucokinase activator (RO-28–1675) for 16 h (n = 2). F, Western blotting analysis of Ago2 and Gck from the liver of 10-week-old WT mice fasted for 16 h (n = 3). G, Western blotting analysis of Ago2 and Gck from the liver of 10-week-old Lepob/ob mice and wild-type (WT) littermates (n = 3). Results are presented as mean ± SEM. *P < 0.05.