Fig. 3.

Nrf2 induction by sterubin. HT22 cells were treated with increasing concentrations of sterubin or eriodictyol for 4 h. Nuclei were prepared and examined by Western blotting for Nrf2 and ATF4. Representative blots are shown (A). (B) and (C) Quantification of the results from 3 independent experiments as shown in (A). * p < 0.05; ** p < 0.01; *** p < 0.001. (D) HT22 cells were treated with 10 µM sterubin for 2–8 h and whole cell extracts examined for the indicated proteins by Western blotting. Similar results were obtained in 3 independent experiments.