Table 2.
Biological activities of sterubin, eriodictyol, homoeriodictyol and fisetin in the five phenotypic screening assays.
| Compound | Protection against oxytosis EC50(µM) | Protection against energy loss EC50(µM) | Anti-inflammatory activity EC50(µM) | Neurotrophic activity EC50(µM) | Intracellular abeta toxicity EC50(µM) | TEAC |
|---|---|---|---|---|---|---|
| Sterubin | 0.8 | 0.9 | 1.4 | 5 | 0.8 | 0.6 |
| Eriodictyol | 2.9 | 1 | 4.1 | > 10 | 2.0 | 0.6 |
| Homoeriodictyol | > 10 | > 10 | > 10 | > 10 | > 10 | 0.08 |
| Fisetin | 3 | 3 | 2.5 | 5 | 3.3 | 3.0 |
The EC50s for the three flavonoids found in Yerba santa (sterubin, eriodictyol, homoeriodictyol) along with fisetin as a positive control in the 5 different phenotypic screening assays as well as their TEAC values, a measure of direct anti-oxidant activity, were determined as described in Section 2.