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. 2018 Dec 27;21(2):185–196. doi: 10.1016/j.neo.2018.09.008

Figure 2.

Figure 2

A549 subtypes feature distinct expression patterns. (A) Principal component analysis showing distinct clustering of holoclonal cultures (1.1, 1.2, 1.5) in green, meroclonal cultures (2.2, 2.3, 2.4) in cyan, paraclonal cultures (3.2, 3.4, 3.7) in purple, and the parental cultures in red. PC1: 61% variance, PC2: 15% variance. (B) Venn diagram showing genes that are dysregulated (P < .05) in the indicated subtypes compared to parental cells. (C) Expression of selected genes. Y-axis represents X-fold change of mean ± SD normalized to parental. (D) Quantification of fluorescence signal intensity (minus background) of selected markers detected by immunofluorescence microscopy. Y-axis represents mean signal intensity ± SD of three subcultures from holoclone (1.1, 1.5, 1.10), meroclone (2.2, 2.3, 2.4), and paraclone cells (3.2, 3.4, 3.7) (n = 80). Ordinary one-way ANOVA was used for significance analysis (*P < .05, **P < .01, ***P < .001, ****P < .0001).