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Immunohistochemical evaluation of Schwann cells and regenerating axons. The immunohistochemical staining (brown reaction) of S-100 was used to reveal the presence and distribution of Sch whereas Gap-43 was used as a marker of newly formed regenerating axons. Both immunostaining were performed in each experimental group (Auto, Nano, and Coll-Nano) and native control (CTR). Scale bar = 100 μm in lower magnifications and 50 μm higher magnification images.
