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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Cell Host Microbe. 2017 Nov 5;22(5):627–638.e7. doi: 10.1016/j.chom.2017.10.003

Figure 6. TRIM25 inhibits the onset of mRNA chain elongation.

Figure 6.

(A) Capped RNA-primed viral mRNA synthesis using vRNPs and globin mRNA as a primer was carried out in vitro. The indicated amounts of human or gibbon TRIM25 (T25) were added to the reaction mixture. RNA products were resolved by electrophoresis on 5% SDS-PAGE. Transcripts were quantified in Image J by measuring band intensities of each lane. The relative amounts of transcripts are shown in a graph below each sample, normalized to the sample containing no TRIM25. (B) 32P-cap-labeled, 42 base-long RNA was incubated with 0.5 ¼g of vRNP in the absence of NTPs (lanes 1-3) or the presence of the four NTPs (lanes 5-7), and where indicated, 3 ¼g of human or gibbon TRIM25. Labeled RNAs were resolved by electrophoresis on 10% SDS-PAGE. The amount of 14 nt RNA primer was quantified in Image J for lanes 1-3 and normalized to the sample containing no TRIM25. Data for panels A and B are representative of two experimental replicates.