Fig. 3.

Model of CENP-A nucleosome dynamics over the cell cycle. In the S phase, centromeric domain replication is accompanied by the dilution of CENP-A proteins and potentially its distribution as a heterotetramer, whereas H3 nucleosomes conserve their octameric structure within the centromere. Then, kinetochore proteins are recruited at the centromere, such as the complex composed of CENP-T, CENP-W, CENP-S, and CENP-X, for the formation of the kinetochore and the correct chromosome segregation. At the exit of mitosis–early G1, newly synthesized CENP-A is incorporated into the chromatin to form an octameric nucleosome. This remodeling process requires the histone chaperone HJURP (Holliday junction recognition protein); still, the exact mechanism is not known