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. 2018 Apr 17;282:120–130. doi: 10.1016/j.jconrel.2018.04.031

Fig. 6.

Fig. 6

PAQ11 intranasal vaccination generated tissue-resident PA-specific CD8+ T cells in the lung. Lung CD8+ T cells were labeled by intravenous anti-Thy1.2 antibody in mice 10 d after secondary PAQ11 vaccination. (A) Representative flow cytometry plots displaying TR CD8+ T cells (“Thy 1.2−”) or circulating (“Thy 1.2+”) CD8+ T cells. (B) Percentages (left) and numbers (right) of TR CD8+ T cells. (C) Representative flow cytometry plots displaying TR marker CD69 expression of TRM CD8+ T cells. (D) Percentages (left) and numbers (right) of CD44hi CD69+ TR CD8+ T cells. (E) Representative flow cytometry plots displaying lung TR (top) or circulating (bottom) PA-specific CD8+ T cells. (F) Percentages (left) and numbers (right) of lung TR (top) or circulating (bottom) PA-specific CD8+ T cells. Each panel is representative of six mice and at least two independent experiments, ****P < 0.0001, ***P < 0.001, **p < 0.01, *P < 0.05, ns: not significant by two-way ANOVA. Dose: I.n. or s.c. administration of 50 μL of 2 mM peptide at day 0 and day 28.