Figure 2:
Two distinct anti-mitsugumin-53 (MG53) antibodies confirm variable localization of MG53 in control human muscle. Sequential muscle cryosections from Control 2 were labelled with either rabbit polyclonal anti-MG53 plus a mouse-monoclonal recognizing γ-sarcoglycan (to demonstrate membrane fidelity in fibers that did not show MG53 sarcolemmal labelling) (top row), or mouse monoclonal anti-MG53 plus rabbit polyclonal anti-MG53 antibodies (bottom row). Both antibodies show a highly similar staining pattern for MG53, which variably localizes to sarcolemma or cytoplasm (asterisks) in different fibers. Bar = 50 μm.