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. Author manuscript; available in PMC: 2019 Apr 11.
Published in final edited form as: Lab Invest. 2018 Oct 11;99(1):138–145. doi: 10.1038/s41374-018-0136-2

Figure 3: Barrier protection by Y-27632 is correlated with a reduction of cellular contractile forces whereas barrier protection by Angpt-1 is not.

Figure 3:

Plotted is gaps versus contraction for the indicated conditions. For gaps, we report changes normalized to vehicle. For contraction, quantities have been normalized to t=0min values and then to the vehicle group. Each treatment group comprises of measurements performed over n=7–16 individual wells of a 96-well plate. Plotted is the mean and standard error. Symbols in red, blue and green correspond to the LPS/TNFα/thrombin, Y-27632 and Angpt-1 treatments, respectively. The ellipses highlight contractile force dependent (blue) or independent (green) reduction of thrombin-, LPS, and TNFα-induced gap formation. A lack of concordance for the EC response to TNFα on the soft substrate remains unclear (Fig. S8).