Antibodies |
ubiquitin |
Enzo Life Sciences |
Cat# ADI-SPA-200-F |
NFE2L1 |
Cell Signaling |
Cat# 8052S |
NFE2L2 |
Santa Cruz |
Cat# sc-365949 |
Cul1 |
Bethyl |
Cat# A303–373A |
GAPDH antibody (FL-335) HRP |
Santa Cruz |
Cat# sc-25778 |
GAPDH |
Cell Signaling |
Cat# 2118S |
XBP1 |
BioLegend |
Cat# 647502 |
CHOP |
Cell Signaling |
Cat# 2895P |
PERK |
Cell Signaling |
Cat# 5683P |
Bip |
Cell Signaling |
Cat# 3177P |
γH2AX |
Millipore |
Cat# 05-636 |
PARP1 |
Cell Signaling |
Cat# 5625S |
IκBα |
Abcam |
Cat# ab32518 |
c-Myc |
Abcam |
Cat# ab32072 |
MDM2 |
Thermo Fischer Scientific |
Cat# PI700555 |
p53 |
Abcam |
Cat# ab1101 |
Chemical, Peptide and
proteins |
Alexa Fluor™ 488 C5 Maleimide |
Thermo Fisher Scientific |
Cat# A10254 |
gliotoxin |
Sigma-Aldrich |
Cat# G9893 |
TNF-α |
Sigma-Aldrich |
Cat# H8916 |
Critical Commercial
Assays |
CellTiter-Glo® Luminescent Cell
Viability Assay |
Promega |
Cat# G7572 |
Cell lines |
HCT116 |
ATCC |
Cat# CCL-247™ |
UbG76V GFP Hela cell line |
T.F. Chou, Harbor-UCLA and LA BioMed |
|
RPE1 WT and BTZ-resistant cell lines |
T.M. Kapoor, The Rockefeller
University |
|
Software |
Graphpad |
Prism |
N/A |