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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Virology. 2018 Jun 20;521:138–148. doi: 10.1016/j.virol.2018.06.004

Figure 2.

Figure 2

ZIKV barcode viruses replicate similar to wild-type clone derived virus and have differences in barcode diversity.

(A–D) The four barcode viruses have similar replication in two mammalian (Vero (A) and LLC-MK2 (B)) and two insect-derived (C6/36 (C) and Aag2 (D)) cell lines. Cells were infected at MOI 0.01. Titers were compared using a two-way ANOVA. E) The number of unique barcodes present in each barcoded virus after transfection (passage 0) or one passage at MOI 0.01 on Vero cells (n=3). Values were compared using a one-way ANOVA for each passage. F) Average genetic complexity at the barcode positions for each barcoded virus. Calculated as -∑i[niN·log2(niN)]. Where ni is the frequency of each nucleotide at each barcode position and N is the total number of barcode calls at that position. Values were compared using a one-way ANOVA for each passage and between passages. **** p<0.0001. G) Frequency of individual barcodes. Each color represents a unique barcode. More colors indicate increased barcode diversity.