Skip to main content
. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Mol Cancer Res. 2017 Aug 29;15(12):1792–1802. doi: 10.1158/1541-7786.MCR-17-0067

Fig. 4. Effects of elevated [cAMP] on metastatic melanoma cells are not dependent on CREB.

Fig. 4.

(A) Metastatic melanoma cells were transduced with CREB shRNA or scrambled shRNA (control) lentivirus and transduced cells were selected using puromycin. Western blot shows CREB knockdown, pCREB and pERK levels 6 days post-transduction. (B) Effect of cAMP elevation by treatment with forskolin (F) on the clonogenicity of control and CREB-knockdown melanoma cells. Forskolin or DMSO (D) was replenished every 72 h for three weeks. Data (mean ± SD) from 3 replicates analyzed by unpaired Student’s t test are shown. P values: * indicates P ≤0.05; ** ≤0.01; *** ≤0.001 and **** ≤0.0001.