Skip to main content
. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: ACS Infect Dis. 2018 May 17;4(8):1211–1222. doi: 10.1021/acsinfecdis.8b00035

Figure 3.

Figure 3.

DNA cleavage induced by GSK000 is mediated by M. tuberculosis gyrase. The bar graph shows results from reactions that contained negatively supercoiled DNA in the presence of M. tuberculosis gyrase (Gyr, black bar), GSK000 (200 μM) in the absence of gyrase (GSK000), or complete reaction mixtures containing 10 μM GSK000 and gyrase that were stopped with SDS prior to the addition of EDTA (blue bar), and reactions that were treated with EDTA (green bar) or NaCl (yellow bar) prior to SDS. Error bars represent the SD of at least 4 independent experiments.