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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: J Immunol. 2017 Dec 18;200(3):1016–1026. doi: 10.4049/jimmunol.1701177

FIGURE 2.

FIGURE 2.

AIM2 deficiency enhances the induction of IFN-β upon BCG infection or stimulation with various DNA forms in BMDCs and BMDMs. BMDCs and BMDMs from WT, cGAS−/− (A), and AIM2−/− mice (B) were infected with BCG (multiplicity of infection = 10) for 6 h, and IFN-β transcripts were measured by quantitative real-time PCR (qRT-PCR). (C) BMDCs and BMDMs from WT and AIM2−/− mice were stimulated with GD (1.5 μg/ml), poly(dA:dT) (1.5 μg/ml), and poly(I:C) (2 μg/ml), and IFN-β was measured by qRT-PCR 8 h after transfections. (D) BMDCs from WTand AIM2−/− mice were stimulated with 0.5, 1, or 2 μg/ml DNA for 10 h, and IFN-β was measured by qRT-PCR and ELISA. Data are representative of at least three independent experiments. The results are shown as mean ± SEM. ***p < 0.001, Student t test. ns, not significant.