Skip to main content
. Author manuscript; available in PMC: 2019 Apr 3.
Published in final edited form as: Nature. 2018 Oct 3;562(7727):444–447. doi: 10.1038/s41586-018-0587-z

Figure 1. The missing link in pilus biogenesis and cryo-EM of FimD-tip complex.

Figure 1.

(a) FimD-tip components. LD: Lectin domain. PD: Pilin domain. N-tail: 24 residues preceding the FimD folded NTD. (b) A partial pilus biogenesis sketch. The red arrows and question marks highlight a key unknown step – the NTD-to-CTDs handover of the chaperone-subunit. DsbA catalyzes disulfide bond formation in a nascent subunit, a prerequisite for subunit recognition by FimC 23. (c,d) The cryo-EM 3D map of Conformer 1 at 4.0 Å resolution (c) and of Conformer 2 at 5.1 Å resolution (d), respectively. Subunits are individually colored as in (a).