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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Carbohydr Res. 2018 May 15;464:19–27. doi: 10.1016/j.carres.2018.05.002

Fig. 3. Preparative HPLC separation of soy protein N-glycan AEAB conjugates.

Fig. 3.

a) the elution profile (UV 330 nm) of 0.8 g N-glycan AEAB conjugates on a preparative NP HPLC (50 × 250 mm amino column); 8 pooled fractions: F1-F8 were collected (F1: 61.1 min to 66.2 min; F2: 66.2 min to 71.1 min; F3: 71.1 min to 73.9 min; F4: 73.9 min to 77.9 min; F5: 77.9 min to 85.2min; F6: 85.2 min to 90.4 min; F7: 90.4 min to 97.8 min; F8: 97.8 min to 109.8 min); b) MALDI-TOF-MS of pooled fractions F1-F8 from the preparative NP-HPLC; c-j) Pooled fractions F1-F8 collected from preparative NP HPLC as annotated in (a) were separated on second dimensional preparative reversed phase HPLC using a 21.2 × 250 mm Luna C18(2) column. Further chromatography was used to purify partially purified glycans when needed.