Table 1. Median, range, mean, and standard deviation of landmark distances for various tissues after linear and linear + non-linear registration.

For each organ and species combination, two serial sections were prepared. Mu = mouse.

	Sample
	Mu Brain	Mu Liver	Mu Spleen	Rat Brain	Rat Kidney	Mean
No. of Point Pairs	25	18	30	30	30	26.6
Median Dist. Linear (μm)	37.1	24.23	73.44	33.99	13.69	36.49
Median Dist. Linear + Non-linear (μm)	8.14	6.44	9.35	9.51	6.52	7.99
Range Dist. Linear (μm)	(7.16, 84.03)	(6.02, 54.24)	(18.81, 164.08)	(3.58, 178.78)	(3.53, 79.53)
Range Dist. Linear + Non-linear (μm)	(1.69, 58.12)	(1.5, 21.21)	(2.54, 41.14)	(2.54, 40.6)	(1.58, 28.5)
Mean Dist. Linear (μm)	37.88 ± 19.93	27.78 ± 16.09	73.7 ± 32.22	46.73 ± 40.33	17.39 ± 14.82	40.69 ± 19.2
Mean Dist. Linear + Non-linear (μm)	12.91 ± 12.36	7.99 ± 4.01	12.07 ± 9.97	11.56 ± 9.1	8.99 ± 6.69	10.7 ± 1.88
Mean Dist. Fold change	2.93 ± 1.61	3.47 ± 4.01	6.11 ± 3.23	4.04 ± 4.43	1.93 ± 2.22	3.7 ± 3.1