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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2018 Aug 13;32(1):68–78. doi: 10.1111/pcmr.12724

Fig. 1.

Fig. 1.

T4H2 transduced T cells produce superior IFNγ responses to gp100209–2M modified peptide in concert with TCR binding affinity. (A) 50,000 CD8 T cells transduced to express the SILv44, T4H2 or R6C12 TCRs were combined 1:1 with peptide-pulsed T2 target cells for 20 hours. IFN-γ levels were measured in the supernatant, with ED50 T4H2<R6C12<SILv44-transduced cells. (B) Binding of T4H2, R6C12, and SILv44 TCRs to gp100209–2M/HLA-A2 measured by SPR. Affinities in terms of KD values are shown in the inset; values are averages and standard deviations of six independent measurements for SILv44 and R6C12 and five independent measurements for T4H2.