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. Author manuscript; available in PMC: 2019 Dec 20.
Published in final edited form as: Mol Cell. 2018 Nov 8;72(6):985–998.e7. doi: 10.1016/j.molcel.2018.10.007

Figure 3: DBC1 recruits PACS-2 to SIRT1.

Figure 3:

(A) FLAG-tagged PACS-2 was co-expressed with Myc-tagged DBC1 and different V5-tagged SIRT1 constructs in HCT116 cells. PACS-2 was immunoprecipitated (FLAG) and bound SIRT1 constructs were detected by western blot (V5). Data are mean ± SD, n = 4. (B) FLAG-tagged PACS-2 was co-expressed with different V5-tagged SIRT1 constructs in HCT116 cells. PACS-2 was immunoprecipitated (FLAG) and bound SIRT1 constructs were detected by western blot (V5), n = 3. (C) Myc-tagged DBC1 was co-expressed with different V5-tagged SIRT1 constructs in HCT116 cells. DBC1 was immunoprecipitated (Myc) and bound SIRT1 constructs were detected by western blot (V5), n = 3.