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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Gastroenterology. 2018 Sep 27;156(1):130–144.e10. doi: 10.1053/j.gastro.2018.09.046

Figure 1.

Figure 1.

Non-dysplastic Barrett’s cells treated with 20 weeks of acidic bile salt exposure (BEC- 20W) demonstrate features of EMT. (A) Immunofluorescent staining for CDH1, FN1, and vimentin. Cell migration without (B) and with (C) a basement membrane (BM) matrix. (D) Cell migration in 3D organotypic culture. Scale bars = 20 µm. For cell migration assays in B &C, bar graphs depict the mean ± SEM of three independent experiments. **p<0.01 vs. BAR-T; ++p<0.01 vs. BAR-10T.