Table 2:
Transcripts (loci) showing significant differences (FDR 1% at isotig level) in expression of mature male gonad tissue between Mytilus edulis (mussels from Swansea, E) and M. galloprovincialis (mussels from Vigo, G), with GO or protein name terms associated with the search term string “SPERM*” OR “FERT*” and a prediction that they have a signal peptide (SP) or a transmembrane (TM) domain in their sequences, this later information coming from SignalP 4.1, TMHMM 2.0 and InterProScan 5.0 analysis. Transcripts were functionally annotated using Blast2GO against UniProt-SwissProt database [all organisms], but protein names below are derived by checking against the nrNCBI[Mollusca] protein database. The numbers of significant isotigs from each locus (FDR 1%) with higher expression levels in M. edulis compared to M. galloprovincialis (E<G) and vice-versa (G>E) are also displayed.
| Transcript # | Gene name | Protein name (nrNCBI [Mollusca]) | Function | SP, TM | N. Isotigs E>G | N. Isotigs G>E |
|---|---|---|---|---|---|---|
| Locus_2854 | Iap2 | Apoptosis 2 inhibitor [C. gigas] | Spermatogenesis, acrosome reaction | TM | 1 | 2 |
| Locus_3972 | Tmbim6 | Bax inhibitor-1 protein [M. galloprovincialis] | Spermatogenesis, acrosome reaction | TM | 3 | 2 |
| Locus_9050 | Bre-4 | Beta-1,4-N-acetylgalactosaminyltransferase bre-4 [C. gigas] | Sperm-egg interaction | TM | 0 | 1 |
| Locus_1384 | CtsB | Cathepsin B [C. ariakensis] | Spermatogenesis, acrosome reaction | SP, TM | 2 | 2 |
| Locus_175 | CtsL | Cathepsin L [C. gigas] | Spermatogenesis, acrosome reaction | SP, TM | 3 | 2 |
| Locus_2547 | 0 | 2 | ||||
| Locus_587 | CtsL2 | Cathepsin L2 cysteine protease [P. fucata] | Spermatogenesis, acrosome reaction | TM | 1 | 1 |
| Locus_6135 | Cdc42 | Cell division cycle 42 [Mytilus sp. ZED-2008] | Sperm capacitation, acrosome reaction | TM | 1 | 0 |
| Locus_24960 | Cht3 | Chitinase-3 [H. cumingii] | Sperm-egg interaction | TM | 0 | 3 |
| Locus_6902 | Cdyl2 | Chromodomain Y-like protein 2 [C. gigas] | Spermatogenesis | TM | 0 | 1 |
| Locus_1290 | Cng | Cyclic nucleotide-gated channel rod photoreceptor sub. alpha [C. gigas] | Spermatogenesis | TM | 0 | 1 |
| Locus_1433 | Dnal1 | Dynein light chain 1, axonemal, partial [C. gigas] | Sperm motility | TM | 0 | 1 |
| Locus_2552 | Eif4g2 | Eukaryotic translation initiation factor 4 gamma 2 [C. gigas] | Spermatogenesis | SP, TM | 1 | 2 |
| Locus_5126 | Ggnbp2 | Gametogenetin-binding protein 2 [C. gigas] | Spermatogenesis | TM | 1 | 3 |
| Locus_134 | Hsp90 | Heat shock protein 90 [M. galloprovincialis] | Spermatogenesis | TM | 1 | 1 |
| Locus_22899 | Prdm9 | Histone-lysine N-methyltransferase PRDM9 [C. gigas] | Spermatogenesis | TM | 0 | 1 |
| Locus_18746 | Suv39h2 | Histone-lysine N-methyltransferase SUV39H2 [C. gigas] | Spermatogenesis | TM | 1 | 1 |
| Locus_6027 | Hya | Hyaluronidase [C. gigas] | Sperm-egg interaction | SP, TM | 1 | 0 |
| Locus_1259 | Irs | Insulin-related peptide receptor [P. fucata] | Spermatogenesis | SP, TM | 1 | 6 |
| Locus_12988 | 1 | 1 | ||||
| Locus_5663 | Ift172 | Intraflagellar transport protein 172 homolog, predicted [A.
californica] |
Sperm motility | TM | 2 | 2 |
| Locus_2244 | Imp2 | Mitochondrial inner membrane protease subunit 2 [C. gigas] | Spermatogenesis | TM | 1 | 2 |
| Locus_10336 | Nphp1 | Nephrocystin-1 [C. gigas] | Spermatogenesis | SP, TM | 0 | 2 |
| Locus_9945 | Pmca | Plasma membrane calcium ATPase [P. fucata] | Sperm motility | TM | 2 | 4 |
| Locus_1143 | Phb | Prohibitin [O. tankahkeei] | Spermatogenesis | TM | 1 | 1 |
| Locus_1157 | Phb2 | Prohibitin-2-like, predicted [A. californica] | Spermatogenesis | TM | 0 | 1 |
| Locus_19017 | Pc1 | Prohormone convertase 1 [H. diversicolor sup.] | Sperm-egg interaction, sperm capacitation, sperm motility | SP, TM | 0 | 2 |
| Locus_2686 | Psma2 | Proteasome subunit alpha type-2 [C. gigas] | Sperm capacitation, acrosome reaction | TM | 0 | 1 |
| Locus_29609 | Rarb | Retinoic acid receptor beta [C. gigas] | Spermatogenesis | SP, TM | 0 | 3 |
| Locus_29136 | Ropn1 | Ropporin-1-like protein [C. gigas] | Spermatogenesis, sperm motility | TM | 0 | 1 |
| Locus_815 | Sqstm1 | Sequestosome-1 [C. gigas] | Spermatogenesis | TM | 0 | 1 |
| Locus_9081 | Slc6a5 | Sodium- and chloride-dependent glycine transporter 2 [C. gigas] | Sperm motility | TM | 1 | 2 |
| Locus_3269 | Slc9c1 | Sodium/hydrogen exchanger 10 [C. gigas] | Spermatogenesis, sperm motility | TM | 2 | 2 |
| Locus_29004 | Spatc1 | Speriolin [C. gigas] | Spermatogenesis | TM | 1 | 5 |
| Locus_13213 | Spa17 | Sperm surface protein Sp17 [C. gigas] | Spermatogenesis, sperm-egg interaction, sperm capacitation, acrosome reaction | TM | 0 | 1 |
| Locus_12286 | Spag1 | Sperm-associated antigen 1 [C. gigas] | Sperm-egg interaction | TM | 1 | 1 |
| Locus_1176 | Srsf4 | Splicing factor, arginine/serine-rich 4 [C. gigas] | Spermatogenesis | SP, TM | 1 | 1 |
| Locus_10277 | 0 | 1 | ||||
| Locus_18976 | Samd7 | Sterile alpha motif domain-containing protein 7 [C. gigas] | Spermatogenesis | TM | 1 | 1 |
| Locus_1959 | Slc26 | Sulfate transporter-like, predicted [A. californica] | Sperm motility | TM | 2 | 2 |
| Locus_4801 | Cct2 | T-complex protein 1 (TCP-1) subunit beta [C. gigas] | Sperm-egg interaction | TM | 1 | 0 |
| Locus_586 | Cct4 | T-complex protein 1 (TCP-1) subunit delta [C. gigas] | Sperm-egg interaction | TM | 0 | 2 |
| Locus_1374 | Cct5 | T-complex protein 1 (TCP-1) subunit epsilon [C. gigas] | Sperm-egg interaction | - | 4 | 3 |
| Locus_24738 | Cct7 | T-complex protein 1 (TCP-1) subunit eta [C. gigas] | Sperm-egg interaction | TM | 0 | 1 |
| Locus_22131 | Cct3 | T-complex protein 1 (TCP-1) subunit gamma [C. gigas] | Sperm-egg interaction | TM | 0 | 2 |
| Locus_25048 | 2 | 2 | ||||
| Locus_36832 | 0 | 1 | ||||
| Locus_20775 | Cct8 | T-complex protein 1 (TCP-1) subunit theta [C. gigas] | Sperm-egg interaction | TM | 2 | 1 |
| Locus_188 | Cct6a | T-complex protein 1 (TCP-1) subunit zeta [C. gigas] | Sperm-egg interaction | - | 1 | 0 |
| Locus_8047 | Thbs1 | Thrombospondin-1 [C. gigas] | Sperm-egg interaction | SP, TM | 0 | 2 |
| Locus_29534 | 0 | 1 | ||||
| Locus_17402 | Ubc8 | Ubiquitin-conjugating enzyme E2–24 kDa [C. gigas] | Spermatogenesis | TM | 1 | 0 |
| Locus_39229 | M3 | vitelline coat lysin M3 [M. edulis] | Sperm-egg interaction | SP | 1 | 1 |
| Locus_25485 | 0 | 1 | ||||
| Locus_24 | M6 | vitelline coat lysin M6 [M. edulis] | Sperm-egg interaction | SP | 1 | 2 |
| Locus_30388 | 0 | 2 | ||||
| Locus_3846 | Zfr | Zinc finger RNA-binding protein [C. gigas] | Spermatogenesis | TM | 1 | 0 |
| Locus_1040 | Zan | Zonadhesin [C. gigas] | Sperm-egg interaction | TM | 1 | 0 |
| Locus_1240 | 1 | 2 | ||||
| Locus_1570 | 1 | 1 | ||||
| Locus_2570 | 0 | 1 |