Table 3:
Identification by MS/MS of 44 out of 45 protein spots (see Fig. 3) from sperm that showed significant differences (q<0.05) between the two analysed species and populations of mussels (M. galloprovincialis from Vigo vs M. edulis from Swansea). Gene, the name of the gene (official gene symbol, retrieved from UniProt) that code for the protein sequence described in “Protein id” column. FC, fold change, defined as the unstandardized effect size with higher expression in either M. galloprovincialis (G) or M. edulis (E) mussel species. The databases from which an identification of the protein spots was obtained are given in the Database column: EST, expression sequence tags from Mytilus spp. available in Genbank, RNA, sequences obtained in the current study and, NCBI, protein sequences from Mollusca available in NCBI (see Materials and Methods).
| Spot | Gene | Protein id | FC | Database | Cellular location | Molecular Function |
|---|---|---|---|---|---|---|
| 1205 | Aco2 | Aconitate hydratase | 1.7 E | RNA, NCBI | Mitochondrion | Tricarboxylic acid cycle, sperm capacitation, motility |
| 1241 | 2.0 E | RNA, NCBI | ||||
| 1272 | Ak | Arginine kinase | 2.0 G | RNA, EST, NCBI | Cytoplasm | Phosphorylation, motility |
| 1744 | 2.1 G | RNA, EST, NCBI | ||||
| 705a | Atp5a | ATP synthase subunit alpha | 1.8 E | RNA, EST, NCBI | Mitochondrion | Respiratory electron transport chain, motility |
| 430 | Npr1 | Atrial natriuretic peptide receptor 1 | 1.7 G | RNA, NCBI | Membrane | Hormone binding, capacitation, chemotaxis |
| 1074 | Cnn1 | Calponin protein | 4.2 G | RNA, EST, NCBI | Cytoskeleton | Actin binding, motility |
| 2151 | Uqcrc2 | Cytochrome b-c1 complex subunit 2 | 1.9 G | RNA, EST | Mitochondrion | Respiratory electron transport chain, motility |
| 2164a | 1.6 G | RNA, EST | ||||
| 847 | 5.6 E | RNA, EST | ||||
| 705b | Dld | Dihydrolipoyl dehydrogenase | 1.8 E | RNA, EST, NCBI | Mitochondrion | Capacitation, acrosome reaction, motility |
| 1119 | Efhc2 | EF-hand domain-containing family member C2 | 4.7 G | RNA, EST, NCBI | Ubiquitous | Calcium binding, acrosome reaction, motility |
| 1134 | 2.4 E | RNA, EST, NCBI | ||||
| 191 | 2.3 G | RNA, EST, NCBI | ||||
| 1536 | Etfb | Electron transfer flavoprotein subunit beta | 1.4 G | RNA, EST, NCBI | Mitochondrion | Respiratory electron transport chain, motility |
| 801 | Eno | Enolase | 1.6 G | RNA, NCBI | Cytoplasm | Glycolysis, motility |
| 1608 | es1 | es1 protein | 2.1 G | RNA, EST | Mitochondrion | Unknown |
| 2039 | 3.5 E | RNA, EST, NCBI | ||||
| 1602 | 2.4 G | RNA, EST | ||||
| 589 | Glud | Glutamate dehydrogenase | 2.0 G | RNA, EST, NCBI | Mitochondrion | Oxidoreductase |
| 2062 | 2.5 E | RNA, EST, NCBI | ||||
| 1265 | Ppa1 | Inorganic pyrophosphatase | 2.6 G | RNA, EST, NCBI | Cytoplasm | Hydrolase, motility, sperm capacitation, acrosome reaction |
| 1094 | Idh3a | Isocitrate dehydrogenase [NAD] subunit alpha | 1.2 E | RNA, EST, NCBI | Mitochondrion | Tricarboxylic acid cycle, motility |
| 1085 | Idh3g | Isocitrate dehydrogenase [NAD] subunit gamma | 3.1 E | RNA, EST, NCBI | Mitochondrion | Tricarboxylic acid cycle, motility |
| 1087 | 1.5 E | RNA, EST | ||||
| 1012 | Ivd | Isovaleryl-CoA dehydrogenase | 2.6 G | RNA, EST, NCBI | Mitochondrion | Aminoacid degradation |
| 988a | Acadm | Medium-chain specific acyl-CoA dehydrogenase | 1.7 G | RNA, EST, NCBI | Mitochondrion | Beta-oxidation |
| 2038 | Sod2 | Mitochondrial manganese superoxide dismutase | 3.4 E | RNA, EST, NCBI | Mitochondrion | Antioxidant, oxidoreductase |
| 949 | Ndufa10 | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 | 4.5 G | RNA, EST | Mitochondrion | Respiratory electron transport chain, motility |
| 2108 | Plc | Perlucin | 1.8 E | RNA | Extracellular region | Shell formation |
| 1919 | Prdx5 | Peroxiredoxin-5 | 2.2 E | EST, NCBI | Mitochondrion | Antioxidant, peroxidase, sperm-egg interaction |
| 1322 | Psme3 | Proteasome activator complex subunit 3 | 1.7 G | RNA, EST | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 1503 | Psma4 | Proteasome subunit alpha type-4 | 1.9 G | RNA, EST, NCBI | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 1795 | Psmb2 | Proteasome subunit beta type-2 | 2.0 G | RNA, EST, NCBI | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 1778 | Psmb6 | Proteasome subunit beta type-6 | 1.6 G | EST, NCBI | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 590 | Pfd0110w | Reticulocyte-binding protein PFD0110w isoform X3 | 1.7 G | RNA, EST | Membrane | Cell-cell adhesion |
| 2164b | Tekt1 | Tektin-1 | 1.6 G | EST, NCBI | Cytoskeleton | Motility |
| 1258 | Tekt2 | Tektin-2 | 1.7 G | RNA, EST, NCBI | Cytoskeleton | Motility |
| 2084 | 2.5 E | RNA, EST, NCBI | ||||
| 776 | 2.3 G | RNA, EST, NCBI | ||||
| 814 | 2.7 G | RNA, EST, NCBI | ||||
| 753 | Tekt4 | Tektin-4 | 1.9 G | RNA, EST, NCBI | Cytoskeleton | Motility |
| 1508 | -- | Uncharacterized protein LOC105318227 | 3.5 G | RNA, EST | -- | -- |
| 1101 | -- | Uncharacterized protein ZK1073.1 isoform X2 | 3.7 G | RNA, EST | -- | -- |
| 988b | Psmc6 | 26S protease regulatory subunit 10B | 1.7 G | EST, NCBI | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 901 | Psmd11 | 26S proteasome non-ATPase regulatory subunit 11 | 1.9 G | RNA, EST, NCBI | Cytoplasm, nucleus | Proteolysis, sperm capacitation, acrosome reaction |
| 1606 | Hsd17b10 | 3-hydroxyacyl-CoA dehydrogenase type-2 | 1.5 E | RNA, EST, NCBI | Mitochondrion | Beta-oxidation, oxidoreductase |
| 97# | 1.7 G |
: due to technical problems this protein spot was not identified by MS. Note that three spots (705, 988 and 2164) were identified as two different proteins (a-b).