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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Anaerobe. 2018 Jun 22;53:108–115. doi: 10.1016/j.anaerobe.2018.06.013

Figure 5. Addition of vanZ1 restores growth of the Δskin mutant in teicoplanin.

Figure 5

Active cultures of strains 630Δerm pMC211 (vector control, MC282), 630Δerm pMC632 (pPcprA::vanZ1, MC907), Δskin + pMC211 (vector control, MC1260), and Δskin pMC632 (pPcprA::vanZ1, MC1261) were diluted in A) BHIS, or with supplementation with B) 0.15 μg/ml teicoplanin, or C) 1.0 μg/ml vancomycin. All cultures also contained 2 μg/ml thiamphenicol to maintain the plasmid and 0.5 μg/ml nisin to induce expression from PcprA. Graphs represent the mean OD600 readings from three independent replicates (Figure S1) with error bars indicating the standard error of the mean. Data were analyzed by two-way ANOVA with Dunnett’s multiple comparison test, comparing to 630Δerm vector control at the same time point. * indicates adjusted P-value < 0.05, ** indicates ≤ 0.001.