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Association of candidate nSOC channels with STIM1 or STIM2 in synaptosomal lysates from WT striatum. Striatal synaptosomal lysates were prepared, solubilized and used in immunoprecipitation experiments with antibodies against Orai1, Orai2, TRPC1, TRPC6, and TRPC4. RabbitIgGwasusedasanegative control for the antibodies. Beads were washed, suspended in SDS loading buffer, boiled and analyzed by western blotting for STIM1 or STIM2.
