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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Cell Microbiol. 2018 Jul 23;21(2):e12886. doi: 10.1111/cmi.12886

Figure 6. The flagellin proteins are decreased in the ΔflaG mutant.

Figure 6.

(A) Immunoblotting analysis of the wild-type, ΔflaG, and ΔflaG+ strains. Equivalent amounts of whole-cell lysates were analyzed by SDS-PAGE and then probed against different antibodies as labeled. PrcA was used as a loading control as previously described (Kurniyati et al., 2017). (B) Protein turnover assay. Spectinomycin was added to the bacterial cultures to arrest protein translation. Samples were withdrawn at the indicated time points and subjected to immunoblotting with antibodies against DnaK, FlaA, or the FlaBs. DnaK was used as a sample loading control.