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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Cell Rep. 2018 Jun 12;23(11):3209–3222. doi: 10.1016/j.celrep.2018.05.028

Figure 4. Effect of Acute Knockdown of L3mbtl1 on Homeostatic Downscaling in Hippocampal CA3 Synapses.

Figure 4.

(A) Homeostatic downscaling induced by PTX treatment for 48 hr in hippocampal CA3 pyramidal neurons. Pyramidal neurons were transfected with expression vectors for shRNA (see Figures S5A and S5B) directed against scramble (middle) and L3mbtl1 (right). Left: representative mEPSC traces; right: averaged mEPSC traces. The shRNAs were transfected at DIV1 and PTX, or DMSO was applied 2 days before recording. mEPSCs were recorded from CA3 pyramidal neurons.

(B) Cumulative distribution of mEPSC amplitudes. KS tests for distributions in untransfected group: ctrl versus PTX, p = 1.8 × 10−31; scaled ctrl versus PTX, not significant. KS tests for distributions in shScr group: ctrl versus PTX, p = 2.1 × 10−26; scaled ctrl versus PTX, not significant. KS tests for distributions in shL3mbtl1 group: ctrl versus PTX, not significant.

(C) Quantification of mean mEPSC amplitudes (left) and frequencies (right).

**p < 0.01, *p < 0.05; ns, not significant; two-way ANOVA test; N = 8 mice were tested. Data shown are means ± SEM.