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In vivo visualization spectral fluorescence imaging of lymphatic drainage of a mouse with five carboxyl Qdots (565, blue; 605, green; 655, yellow; 705, magenta; 800, red) injected intracutaneously into the middle digits of the bilateral upper extremities, the bilateral ears, and at the median chin. Five primary draining lymph nodes are simultaneously demonstrated with different colors through the skin in the in vivo image.
