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. 2018 Dec 21;12:106. doi: 10.3389/fncir.2018.00106

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Copyright © 2018 Hökfelt, Barde, Xu, Kuteeva, Rüegg, Le Maitre, Risling, Kehr, Ihnatko, Theodorsson, Palkovits, Deakin, Bagdy, Juhasz, Prud’homme, Mechawar, Diaz-Heijtz and Ögren.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dark-field ISH photomicrographs showing the distribution of transcripts for tyrosine hydroxylase (TH), galanin, and GalR3 in the locus coeruleus. The three markers TH (A,B), galanin (C,D), and GalR3 (E,F) show overlapping distribution patterns. TH and GalR3 transcript levels seem approximately similar in all cells. In contrast, there is variability in the strength of the signal for galanin mRNA (white arrow points to neurons with a strong signal, red ones to such with a weak signal). Exposure time: TH, 10 days; galanin, 4 weeks; GalR3, 8 weeks. This difference in exposure time transcript reflects difference in transcript levels, that is GalR3 mRNA levels are very low. Reprinted from Le Maitre et al. (2013) [Scale bars: 200 μM for (A,C,E); 50 μM for (B,D,F)].