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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Biochemistry. 2011 Aug 2;50(33):7208–7217. doi: 10.1021/bi200742a

Figure 5.

Figure 5

Effect of mutant GTPases on the targeting and integration of LHCP into thylakoid membranes. LHCP-dp1 and -dp2 denote the two 18.5 kDa and 21 kDa protease-protected fragments that represent LHCP successfully targeted and integrated into the thylakoid membrane. pre-LHCP was added to the reaction after the protease treatment and served as a loading control. (A, B) LHCP integration efficiency by the individual cpFtsY and cpSRP54 mutants, respectively. The top panels show representative data, and the lower panels show quantification of two or more measurements. All the data were normalized to that of the wildtype protein, which was set to 100%. (C, D) Correlation of the translocation defect of cpFtsY (part C) and cpSRP54 (part D) mutants with their kcat/Km values.