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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Behav Brain Res. 2018 Jul 24;359:836–844. doi: 10.1016/j.bbr.2018.07.019

Figure 1.

Figure 1

Schematic of experimental design. Adolescent mice (PND 33-35) were implanted with mini-osmotic pumps to deliver 3.0mg/kg/d nicotine (nic) or saline (sal) for 2 weeks. After pump removal, a 2-week washout period was given. Adult (PND 61-63) mice that previously received sal were re-exposed either to chronic sal or nic (6.3mg/kg/d) for another 2 weeks. Likewise, adolescent nic-exposed mice received either sal or nic (6.3 mg/kg/d) during adulthood. PR testing to examine saccharin and nicotine reinforcement (see Methods) was conducted following the second 2-week washout period. The autoshaping and pretraining for PR testing was initiated in animals moving into the second week of re-exposure.