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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Virology. 2018 Jun 25;521:169–174. doi: 10.1016/j.virol.2018.06.008

Fig. 1.

Fig. 1

Pom121 supports HIV-1 replication. 293T cells were seeded in 24 well plate(1.5 × 105/well) and trans-fected with Pom121 specific siRNA (100 nM). (A) The Pom121 protein level was measured by western blot 72 h post transfection; or cells were infected with HIV-Luc (1.5 ng p24/well) 24 h post transfection and (B) The luci-ferase activity in cell lysis was measured 72 h post infection. TZM-b1 cells were seeded in 24-well plates (1.0 × 105/well) and transfected with Pom121 specific siRNA in 100 nM concentration. (C) The Pom121 protein level was measured by Western blot 72 h post transfection; or cells were infected with HIV-1NLAD8 (1.8 ng p24/well) 24 h post transfection and (D) The luciferase activity in cell lysis was measured 72 h post infection. Data were presented as mean standard deviations from three independent experiments. *P < 0.05, **P < 0.01.