Fig. 4.

Pom121 FG-domain is required for HIV-1 cDNA nuclear import. (A) Schematic of pFlag-Pom121^R, Pom121^dHelix_R and Pom121^dFG structure. The amino acids sequence XFXFG is the motif of FG repeats. The site of siRNA targeting is indicated as arrow. 293T cells were seeded in 24-well plates (1.5 × 10⁵/well) and transfected with indicated siRNA and plasmids to regulate the cellular Pom121 expression. (B)The protein level of endogenous Pom121 or exogenous Pom121^R, Pom121^dHelix_R Pom121^dFG was examined by Pom121 and Flag antibody 72 h post transfection, respectively. (C) The cells were infected with HIV-Luc (1.5 ng/well) 24 h post-transfection and the luciferase activity in infected cell lysis was measured 72 h post-infection. Data were presented as mean standard deviations from three independent experiments. **P < 0.01.