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. 2018 Dec 21;9:3221. doi: 10.3389/fmicb.2018.03221

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Copyright © 2018 Nekouei, Vanderstichel, Ming, Kaukinen, Thakur, Tabata, Laurin, Tucker, Beacham and Miller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Predicted means of loads for the common infectious agents (Y-axes) detected in 2,006 out-migrating Fraser River Sockeye salmon, by study region (X-axes) and year, based on linear regression models. Sampling regions: (1) Freshwater, (2) Strait of Georgia, (3) Discovery Islands, and (4) Johnston Strait and north. Small letters used on the plots represent statistically significant Bonferroni groups, where differences were observed when the points (i.e., unique combinations of region and year) do not have any letters in common (P < 0.05). If the points shared at least one letter with each other, they were not statistically different (P > 0.05). Saltwater agents are presented in the right column, which included regions 2–4. A square root transformation was conducted on the loads of P. theridion, P. minibicornis, P. kabatai, ENV, and gill chlamydia in order to meet the normality and homoscedasticity assumptions in their respective linear regression models. The average loads for these agents have been back-transformed to the original scale. Presented 95% confidence interval for each point was calculated using delta method in Stata v15.1.