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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: J Pathol. 2017 Oct 3;243(3):390–400. doi: 10.1002/path.4960

Figure 1. Human glomerular cell monocultures in 3D.

Figure 1.

(A) ULEX staining of human GEC microvessel networks in the 3D matrix at × 40 power. Inset: lumenisation of GEC cords revealed by GFP lentivirus-transduced GECs in triculture (MCs and podocytes not labelled), showing coalescence of vacuoles to form lumen. (B) Scanning electron microscopy (SEM) of GEC microvessel, showing the lumen (arrow) and (C) abluminal surface with fenestra (arrow). (D) SEM of a MC in the 3D matrix. (E) SEM of podocytes in the 3D matrix. (F) High magnification SEM view of podocyte foot process in the 3D matrix.