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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: J Pathol. 2017 Oct 3;243(3):390–400. doi: 10.1002/path.4960

Figure 3. Responses to TGFβ of human glomerular endothelial cell microvessels and podocytes in 3D.

Figure 3.

(A) Quantification of GEC network arborisation with TGFβ (10 ng/ml) or BMP7 (100 ng/ml) treatment and co-treatment, as assessed by cord number, total cord length, average cord length and branching points (n=3, standard deviation bars, 2-tailed Student’s t test). (B) CTGF mRNA level in podocytes in 2D (black bars) and 3D (white bars) by quantitative reverse transcriptase PCR analysis in response to TGFβ (10 ng/ml) and BMP7 (100 ng/ml) stimulation and co-stimulation (fold change compared to control, n=3, standard deviation bars, 2-tailed Student’s t test).