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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Neurobiol Aging. 2010 Nov 26;33(2):422.e11–422.e25. doi: 10.1016/j.neurobiolaging.2010.09.017

Fig. 2.

Fig. 2.

Identification of CEBPD regulated genes in U373MG cells. (A) U373MG cells stably transfected with zinc-inducible CEBPD expression vector were exposed to 100 μM ZnSO4 for 8 hours and expression of HA-tagged CEBPD protein was analyzed by Western blotting (left panel). Clustering of microarray data from ribonucleic acid (RNA) of cells as shown in panel (A) identified significant gene expression clusters resulting from CEBPD overexpression (right panel). (B) Transcript levels of 11 differentially expressed genes, which code for membrane or secreted proteins, were assessed by reverse transcription-polymerase chain reaction (RT-PCR) analysis. GAPDH served as the loading control. (C) U373MG cells were treated with 20 ng/mL tumor necrosis factor alpha (TNF-α) or 5 ng/ml interleukin (IL)-1β for 3 hours and RT-PCR of indicated genes was performed.