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. 2018 Dec 20;72(6):970–984.e7. doi: 10.1016/j.molcel.2018.10.011

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

SPT6 Depletion Causes H3K36me3 Redistribution

(A) Western blots with indicated antibodies 60 hr post-siSPT6 transfection (versus siLuc nonspecific control). EXOSC3 and H3 profiles shown as loading controls.

(B) Meta-analysis of reads density (FPKM) for ratio of mNuc-seq/H3K36me3 with H3 at TSS of divergent PROMPT-pre-mRNA and enhancer RNA (eRNA) following SPT6 depletion. All subsequent transcription images employing siSPT6 versus siLuc are shown in red and blue, respectively.

(C) Scatterplots of H3K36me3/H3 on pre-mRNA (0 to +3 kb), PROMPT (−3 kb to 0), and eRNA (−3 kb to +3 kb) regions in siLuc versus siSPT6. The percentage of upregulated regions by SPT6 depletion are indicated.

(D) Boxplots of mNuc-seq/H3K36me3 ratio across pre-mRNA gene bodies, PROMPTs (3 kb from TSS), and eRNA (2 kb from center) and across lincRNA gene bodies.

(E) Model of redistributed H3K36me3 marks caused by SPT6 depletion.

See also Figure S2.