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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Microbes Infect. 2018 Jun 26;21(1):50–55. doi: 10.1016/j.micinf.2018.06.003

Fig. 2. LL-37 induction of inflammatory cytokines in human endometrial epithelial cells is inhibited by chlamydial Pgp3.

Fig. 2

The endometrial epithelial cell line MS74 was set up at 1.5 × 105 cells/ml/well in a 24 well plate and the cells were treated with or without 12μM of LL-37, Pgp3 or Pgp3/LL-37 complexes for 24h at 37°C. The supernatants were collected for measuring IL-6 (solid bar) and IL-8 (open) and the results were calculated from 3 independent experiments each with duplicates and displayed as ng/ml along the Y-axis (mean plus/minus standard error). Note that although LL-37 induced significant levels of IL-6 & IL-8, the LL-37 proinflammatory activity was blocked by forming complexes with Pgp3 (**p<0.01 (Wilcoxon ran sum). The Pgp3/LL-37 complexes were formed by pre-incubating the two together each at 12μM in cell culture medium for 30min at 37°C before applying the co-incubation mixtures to the cell culture wells.