Figure 4.

Basic nanopore signal processing. Example translocations of a model protein. (a) Representative current trace (I) as a function of time (t). Each drop in current is defined as a nanopore event. (b) The time between the start of each event is defined as the inter-event interval (t_i), which is fitted by an exponential decay function. (c) The event dwell time (t_D) histogram is fitted by either drift-diffusion model or exponential decay function. (d) The ratio between event amplitude (i_b) and open pore current (i_o) is fitted by multimodal distribution. (e) The scatter plot of I_B vs t_D is used to perform single-molecule protein classification.