Figure 2. Developmental Defect of Embryos.

(A) Rnaseh2a^+/+, Rnaseh2a^RED/RED, Rnaseh2a^RED/G37S, Rnaseh2a^RED/−, and Rnaseh2a^−/− embryos at embryonic days 8.5–11.5. Scale bar, 1 mm.

(B) Survival of mouse embryos from crosses of Rnaseh2a^RED/+ × Rnaseh2a^RED/+ (top) and Rnaseh2a^RED/+ × Rnaseh2a^G37S/+ (bottom). Expected number of Rnaseh2a^RED/RED and Rnaseh2a^RED/G37S at Mendelian ratio is shown in right column.

(C) Rnaseh2a^+/+ and Rnaseh2a^G37S/− embryos and neonates. Scale bars, 2 mm (E14.5) and 1 cm (birth).

(D) Partial rescue of embryonic lethality by ablation of p53 gene. Images of E10.5 embryos are shown. A morphological rescue of Rnaseh2a^RED/RED and Rnaseh2a^RED/G37S was observed on p53 background. Rnaseh2a^RED/G37S was also rescued on p53^+/− background (n = 5), whereas Rnaseh2a^RED/RED was not (n = 3). No rescue of Rnaseh2a^RED/RED (n = 8) and Rnaseh2a^RED/G37S (n = 5) phenotypes was observed on Sting^−/− background.