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Evaluation of on-chip dry reagents’ shelf life. (A) RT-LAMP of MS2 in a multifunctional chip after 180 days of storage. Inset: a photograph of a test (positive control) and a negative (no target) control after 6 months of storage. (B) The threshold time T1/2 for MS2 RNA (0.5 fg, ~ 270 copies) as a function of storage duration (weeks) in a multifunctional chip stored at ambient conditions (blue rhombus) and benchtop (red squares) operating with wet reagents stored at −20 °C.
