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. Author manuscript; available in PMC: 2018 Dec 28.
Published in final edited form as: Sci Signal. 2018 Jun 12;11(534):eaaq0918. doi: 10.1126/scisignal.aaq0918

Fig. 1. SOCE activates a Cl-conductance in ICC.

Fig. 1.

(A) ICC were dialyzed with internal solution V (Table 1; ECl = −40 mV), held at −80 mV, and pretreated with CPA (10 μM) in Ca2+-free external solution (solution II) for 10 min. Inward current was activated when 2 mM Ca2+ (CaPSS, solution I) was restored. Ramp potentials from −80 to +80 mV were run before and after addition of 2 mM Ca2+ (examples noted by • and •). (B) Responses to ramp potentials in (A) [• (black trace) is in 0 mM Ca2+, and • (red trace) is after addition of 2 mM Ca2+]. The current activated by 2 mM Ca2+ was outwardly rectifying and reversed at ECl when corrected for junction potential. (C) SMCs were dialyzed with internal solution V (Table 1; ECl = −40 mV), held at −80 mV, and pretreated with CPA (10 μM) in Ca2+-free external solution (solution II) for 10 min. Ramp potentials from −80 to +80 mV were run before and after addition of 2 mM Ca2+ (examples noted by • and •). (D) Responses to ramp potentials in (C) [• (black trace) is in 0 mM Ca2+, and • (red trace) is after addition of 2 mM Ca2+]. Small inward current after addition of 2 mM Ca2+ is voltage-dependent Ca2+ current common in SMCs. (E) Summary data showing currents induced by reintroducing 2 mM [Ca2+]o at 0 mV in ICC and SMCs. Data are means ± SEM (n = 5 cells for each group; **P < 0.01, ***P < 0.001, Student’s two-tailed t test).