Fig. 6. CRAC current induced by IP₃ in ICC.

NPPB (30 μM) was added to CaPSS (solution I) to block Ca²⁺-activated Cl⁻ current. IP₃ (30 μM) was included in the Cs⁺-rich pipette solution also containing BAPTA (solution VI) to cause depletion of ER Ca²⁺. ICC in the whole-cell configuration were held at −50 mV. (A) Representative trace showing the effect of IP₃ in the presence or absence of GSK-7975A on inward current. Ramp potentials from −80 to +60 mV were run during recording. (B) Responses to ramp potentials in (A) [• (black trace) is control, • (red trace) is with IP₃ dialysis, and • (green trace) is with IP₃ dialysis and GSK-7975A treatment]. (C) Summary data showing the effects of IP₃ and GSK-7975A on CRAC currents. Data are means ± SEM (n = 5 cells for each group; ***P < 0.001 compared to control, ^###P < 0.01 compared to IP₃, one-way ANOVA).